Monoclonal antibodies to human immunodeficiency virus and uses thereof
AssigneeAbbott Laboratories
InventorsSheng C. Lou, Jeffrey C. Hunt, John G. Konrath, Xiaoxing Qiu, James W. Scheffel, Joan D. Tyner
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Abstract
The present invention relates to novel monoclonal antibodies which may be used in the detection of Human Immunodeficiency Virus (HIV). These antibodies exhibit an unusually high degree of sensitivity, a remarkably broad range of specificity, and bind to novel shared, non-cross-reactive epitopes. In particular, the monoclonal antibodies of the present invention may be utilized to detect HIV-1 antigen and HIV-2 core antigen in a patient sample.
Claims7
A method for detecting the presence of one or more antigens selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in a test sample suspected of containing one or more of said antigens, comprising the steps of:
- a) contacting said test sample with at least one monoclonal antibody which binds to a shared epitope of Human Inununodeficiency Virus-1 protein p24 and Human Immunodeficiency Virus-2 protein p26, wherein said at least one monoclonal antibody is selected from the group consisting of 120A-270 produced by hybridoma cell line PTA-3809, 115B-151 produced by hybridoma cell line PTA-2809, 117-289 produced by hybridoma cell line PTA-2806, 103-350 produced by hybridoma cell line PTA-2808, 115B-303 produced by hybridoma cell line PTA-2810 and 108-394 produced by hybridoma cell line PTA-2807, for a time and under conditions sufficient for the formation of antibody/antigen complexes;
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- b) detecting said complexes, presence of said complexes indicating presence of at least one antigen selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in said test sample.
A method for detecting the presence of one or more antigens selected from the group consisting of HIV-1 antigen and HIV-2 antigen, in a test sample suspected of containing one or more of said antigens, comprising the steps of:
- a) contacting said test sample with at least one monoclonal antibody which binds to a shared epitope of Human Immunodeficiency Virus-1 protein 24 and Human Immunodeficiency Virus-2 protein p26, wherein said at least one monoclonal antibody is selected from the group consisting of 120A-270 produced by hybridoma cell line PTA-3890, 115B-151 produced by hybridoma cell line PTA-2809, 117-289 produced by hybridoma cell line PTA-2806, 103-350 produced by hybridoma cell line PTA-2808, 115B-303 produced by hybridoma cell line PTA-2810 and 108-394 produced by hybridoma cell line PTA-2807, for a time and under conditions sufficient for the formation of antibody/antigen complexes;
- b) adding a conjugate to the resulting antibody/antigen complexes for a time and under conditions sufficient to allow said conjugate to bind to the bound antigen, wherein said conjugate comprises an antibody attached to a signal generating compound capable of generating a detectable signal;
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- c) detecting presence of antigen which may be present in said test sample by detecting a signal generated by said signal generating compound, presence of said signal indicating presence of at least one antigen selected from the group consisting of HIV-1 antigen and HIV-2 antigen in said test sample.
A method for detecting the presence of one or more antigens selected from the group consisting of HIV-1 antigen and HIV antigen, in a test sample suspected of containing one or more of said antigens, comprising the steps of:
- (a) contacting:
- 1) at least one monoclonal antibody which binds to a shared epitope of HIV-1 p24 antigen and HIV-2 p26 antigen bound to a solid support, wherein said at least one monoclonal antibody is selected from the group consisting of 120A-270 produced by hybridoma cell line PTA-3890, 115B-151 produced by hybridoma cell line PTA-2809, 117-289 produced by hybridoma cell line PTA-2806, 103-350 produced by hybridoma cell line PTA-2808, 115B-303 produced by hybridoma cell line PTA-2810 and 108-394 produced by hybridoma cell line PTA-2807, 2) said test sample, and 3) an indicator reagent comprising an antibody which binds to HIV-1 antigen and HIV-2 antigen to which a signal generating compound is attached, to form a mixture;
- (b) incubating said mixture for a time and under conditions sufficient to form antibody/antigen/antibody complexes;
- (c) detecting presence of a measurable signal generating by said signal-generating compound, presence of said signal indicating presence of one or more antigens in said test sample selected from the group consisting of HIV-1 antigen and HIV-2 antigen.
Dependencies are inferred from the claim text.