Methods for encapsulating plasmids in lipid bilayers
AssigneeInex Pharmaceuticals Corporation
InventorsJeffery J. Wheeler, Michael Hope, Pieter R. Cullis, Marcel B. Bally
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Abstract
Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are serum-stable.
Claims20
A nucleic acid-lipid particle, said particle comprising a cationic lipid;
- a non-cationic lipid;
- a PEG-lipid conjugate;
- and a nucleic acid.
A pharmaceutical composition comprising a nucleic acid-lipid particle comprising a cationic lipid;
- a non-cationic lipid;
- a PEG-lipid conjugate;
- and a nucleic acid;
- and, a pharmaceutically acceptable carrier.
A method of introducing a nucleic acid into a cell, said method comprising contacting said cell with a nucleic acid-lipid particle comprising a cationic lipid, a non-cationic lipid, a PEG-lipid conjugate, and a nucleic acid.
Dependencies are inferred from the claim text.