Back to results
US 6,815,203 B1Granted
Methods of making pancreatic islet cells
AssigneeJoslin Diabetes Center, Inc.
InventorsSusan Bonner-Weir, Monica Taneja
Life of this patent
Estimating the term…
Abstract
The invention features methods of promoting dedifferentiation of pancreatic cells, methods of obtaining pancreatic islet cells from the dedifferentiated pancreatic cells, and methods of treating a subject having a disorder characterized by insufficient pancreatic islet function by administering pancreatic islet cells obtained by these methods.
Claims36
A method of obtaining pancreatic islet cells, the method comprising:
- providing differentiated pancreatic duct or exocrine cells substantially free of islet cells,
- allowing said differentiated duct or exocrine cells to proliferate to form a population of dedifferentiated pancreatic cells;
- adding a component of extracellular matrix (ECM) to the population of dedifferentiated pancreatic cells;
- and
- growing the cells in the presence of the component of ECM for a time sufficient for the dedifferentiated cells to express insulin, thereby obtaining pancreatic islet cells.
2 more elements
A method of obtaining pancreatic islet cells, the method comprising:
- (a) obtaining a population of adult or differentiated pancreatic cells substantially free of islet cells,
- (b) allowing the adult or differentiated pancreatic cells to proliferate to form a population of dedifferentiated pancreatic cells;
- (c) adding a component of extracellular matrix (ECM) to the population of dedifferentiated pancreatic cells;
- and
- (d) growing the cells in the presence of the component of ECM for a time sufficient for the dedifferentiated cells to express insulin, thereby obtaining pancreatic islet cells.
2 more elements
Dependencies are inferred from the claim text.