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US 6,815,190 B1Granted
Cutinase variants
AssigneeNovozymes A/S
InventorsMasanobu Abo, Shiro Fukuyama, Allan Svendsen, Tomoko Matsui
Life of this patent
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Abstract
The present invention relates to cutinase variants having improved thermostability. The variants have one or more amino acid substitutions within the vicinity of the N-terminal amino acid.
Claims27
A variant of a parent Humicola insolens DSM 1800 cutinase which variant:
- (a) has 1-20 alterations within the amino acid sequence of the parent;
- (b) comprises substitution of one or more amino acid residues at a position which is located (i) within 17 Å from the location of the N-terminal amino acid (as calculated from amino acid residues in a crystal structure), and/or (ii) within 20 positions from the N-terminal amino acid;
- and
- (c) is more thermostable than the parent cutinase.
1 more element
A variant of a parent Humicola insolens DSM 1800 cutinase wherein said variant is more thermostable than the parent cutinase and wherein said variant has 1-20 alterations within the amino acid sequence of the parent which variant comprises a substitution selected from the following substitutions:
- (a) a substitution of an amino acid position E6, E10, E30, E47, D63 and/or E82 in the amino acid sequence of the cutinase of Humicola insolens strain DSM 1800 with a neutral amino acid;
- (b) a substitution of an amino acid at position E179 in the amino acid sequence of the cutinase of Humicola insolens strain DSM 1800 with a neutral amino acid;
- (c) a substitution of an amino acid at position N7, S11, N44 and/or N52 in the amino acid sequence of the cutinase of Humicola insolens strain DSM 1800 with a positive amino acid;
- and
- (d) a substitution of an amino acid position A14 and/or R51 in the amino acid sequence of the cutinase of Humicola insolens strain DSM 1800 with Pro.
2 more elements
Dependencies are inferred from the claim text.