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US 6,812,023 B1Granted
Methods of producing membrane vesicles
AssigneeAnosys, Inc.
InventorsHenry Lamparski, Curtis Ruegg, Jean-Bernard Le Pecq, Di-Hewi Hsu, Jenq Yuan Yao
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AssigneeAnosys, Inc.
InventorsHenry Lamparski, Curtis Ruegg, Jean-Bernard Le Pecq, Di-Hewi Hsu, Jenq Yuan Yao
Estimating the term…
The present invention relates to methods of preparing biological material, for use in various experimental, diagnostic or therapeutic uses, including immunotherapy treatment or prophylaxy of tumors. More particularly, the present invention relates to methods of preparing membrane vesicles (in particular exosomes) released by various types of mammalian cells, comprising diafiltration and/or density cushion centrifugation. The invention also provides novel methods for characterizing and analyzing exosome preparations, which can be used in quality control assay for the purpose of pharmaceutical product production. The invention is suitable to produce pharmaceutical grade preparations of such membrane vesicles and to fully characterize said preparations, for use in human beings.
A method of preparing membrane vesicles, comprising:
The method of claim, 1, further comprising a diafiltration of the preparation from step c.
A method for preparing membrane vesicles, comprising:
Dependencies are inferred from the claim text.