Method of identifying inhibitors of EBNA-1
AssigneeWisconsin Alumni Research Foundation
InventorsWilliam M. Sugden, Atsushi Jun Komano, Gregory Kennedy
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Abstract
A method of assaying potential inhibitors of EBNA-1 is disclosed. In one aspect, this invention comprises the steps of (a) obtaining an EBV-positive cell line and an EBV-negative cell line; (b) exposing the cell lines to a test compound, and (c) observing the effect of the compound on induction of apoptosis in the cell lines, wherein significant apoptosis in the EBV-positive cell line and lack of significant apoptosis in the EBV-negative cell line indicates a compound that specifically inhibits EBNA-1.
Claims9
A method of assaying potential inhibitors of EBNA-1 comprising the steps of:
- (a) obtaining an EBV-positive cell line and an EBV-negative cell line wherein both cell lines are proliferative B-lymphocyte cell lines;
- (b) treating the cell lines with a test compound by exposure of the cells to the test compound in solution or to the test compound as expressed or provided by an intracellular gene construct, and
- (c) examining either cell proliferation or induction of apoptosis in the cell lines, wherein significant apoptosis or decrease in cell proliferation in the EBV-positive cell line and lack of significant apoptosis or increase in cell proliferation in the EBV-negative cell line indicates that the compound specifically inhibits EBNA-1.
Dependencies are inferred from the claim text.