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US 6,809,235 B2Granted
Heat stable mutants of starch biosynthesis enzymes
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsL. Curtis Hannah, Thomas W. Greene
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AssigneeUniversity of Florida Research Foundation, Inc.
InventorsL. Curtis Hannah, Thomas W. Greene
Estimating the term…
The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.
A purified polynucleotide comprising a nucleotide sequence encoding a full-length mutant polypeptide of maize ADP-glucose pyrophosphorylase large subunit, or a fragment of said full-length mutant polypeptide, wherein said full-length mutant polypeptide or said fragment of said full-length mutant polypeptide comprises an amino acid mutation in the amino acid sequence of said maize ADP-glucose pyrophosphorylase large subunit, and wherein when said full-length mutant polypeptide or said fragment of said full-length mutant polypeptide is expressed to form a mutant ADP-glucose pyrophosphorylase enzyme, said mutant enzyme exhibits increased heat stability relative to the wild type ADP-glucose pyrophosphorylase enzyme.
Dependencies are inferred from the claim text.