Controlling plant pathogens with fungal/bacterial anatagonist combinations
OwnerThomas D. Johnsoninventor
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Abstract
Fungal/bacterial atagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal/bacterial antagonist combination and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide.
Claims30
An agricultural inoculum suitable for inoculating plant seeds, said inoculum comprising:
- a fungal antagonist selected from the group consisting of Trichoderma virens isolate (ATCC 58678) and mutants thereof;
- a bacterial antagonist selected from the group consisting of Bacillus subtilis var. amyloliquifaciens strain (ATCC BAA-390) and mutants thereof;
- and
- a suitable carrier that is non-phytotoxic, non-bacteriostatic, and non-bacteriocidal.
A composition of matter comprising:
- a plant seed inoculated with a combination comprising a fungal antagonist selected from the group consisting of Trichoderma virens (ATCC 58678) and mutants thereof and a bacterial antagonist selected from the group consisting of Bacillus amyloliquefaciens (ATCC BAA-390) and mutants thereof;
- wherein said combination suppresses growth of plant pathogenic fungi.
A plant inoculated with a combination comprising:
- a fungal antagonist selected from the group consisting of Trichoderma virens (ATCC 58678) and mutants thereof;
- and
- a bacterial antagonist selected from the group consisting of Bacillus amyloliquefaciens (ATCC BAA-390) and mutants thereof;
- wherein the combination suppresses growth of plant pathogenic fungi and the plant is selected from the group consisting of
- corn,
- sunflower,
- soybean,
- field pea, and
- wheat.
A method of protecting a plant from disease caused by a plant pathogenic fungus comprising:
- inoculating seeds from said plant with a combination comprising a fungal antagonist selected from the group consisting of Trichoderma virens (ATCC 58678) and mutants thereof and a bacterial antagonist selected from the group consisting of Bacillus amyloliquefaciens (ATCC BAA-390) and mutants thereof;
- wherein said combination suppresses growth of plant pathogenic fungi.
A method for biologically controlling or inhibiting stalk rot or root rot comprising:
- coating seeds with an effective amount of a composition comprising Trichoderma virens (ATCC 58678) and Bacillus amyloliquefaciens (ATCC BAA-390).
A process for making a composition comprising:
- introducing an essentially pure culture of Bacillus amyloliquefaciens (ATCC BAA-390) to a growth medium about eight hours after an essentially pure culture of Trichoderma virens (ATCC 58678) is introduced to the growth medium;
- and
- growing the culture as a competitive culture.
A process comprising making a composition comprising:
- combining an essentially pure culture of Trichoderma virens (ATCC 58678) with an essentially pure culture of Bacillus amyloliquefaciens (ATCC BAA-390) in a 50:50 mixture;
- and
- applying said composition to a seed at a rate of at least 100,000 spores per seed.
A composition of matter made by combining:
- a fungal antagonist selected from the group consisting of Trichoderma virens isolate (ATCC 58678) and mutants thereof;
- a bacterial antagonist selected from the group consisting of Bacillus subtilis var. amyloliquifaciens strain (ATCC BAA-390) and mutants thereof;
- and
- a suitable carrier that is non-phytotoxic, non-bacteriostatic, and non-bacteriocidal.
An antagonist for controlling plant pathogens made by combining effective amounts of:
- a fungal antagonist selected from the group consisting of Trichoderma virens isolate (ATCC 58678) and mutants thereof;
- a bacterial antagonist selected from the group consisting of Bacillus subtilis var. amyloliquifaciens strain (ATCC BAA-390) and mutants thereof;
- and
- a suitable carrier that is non-phytotoxic, non-bacteriostatic, and non-bacteriocidal.
A process comprising:
- making a composition by combining an essentially pure culture of Trichoderma virens (ATCC 58678) with an essentially pure culture of Bacillus amyloliquefaciens (ATCC BAA-390) in a mixture;
- and
- applying said composition to a seed;
- wherein said mixture ranges in composition from 10 to 90 percent Trichoderma virens (ATCC 58678) by culture volume and from 90 to 10 percent Bacillus amyloliquefaciens (ATCC BAA-390) by culture volume.
A process comprising:
- making a composition by combining an essentially pure culture of Trichoderma virens (ATCC 58678) with a plurality of essentially pure cultures of bacteria in a mixture;
- and
- applying said composition to a seed;
- wherein said mixture ranges in composition from 10 to 90 percent Trichoderma virens (ATCC 58678) by culture volume.
Dependencies are inferred from the claim text.