Process for the preparation of L-amino acids by fermentation and nucleotide sequences coding for the accDA gene
AssigneeDegussa AG
InventorsYvonne Tilg, Bernhard Eikmanns, Lothar Eggeling, Hermann Sahm, Bettina Möckel
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Abstract
The invention relates to nucleotide sequences coding for the accDA gene and to a process for the preparation of L-amino acids, especially L-lysine, by fermentation using corynebacteria in which the accDA gene is amplified.
Claims20
A process for the production of an L-amino acid comprising:
- culturing recombinant coryneform bacteria under conditions suitable for overexpression of the accDA gene having the nucleic acid sequence comprising SEQ ID NO:
- 1, and wherein said bacteria produce said L-amino acid selected from the group consisting of L-lysine, L-aspartic acid, L-asparagine, L-homoserine, L-threonine, L-isoleucine, and L-methionine.
A process for the production of L-amino acids selected from the group consisting of L-lysine, L-aspartic acid, L-asparagine, L-homoserine, L-threonine, L-isoleucine, and L-methionine comprising:
- a) culturing recombinant coryneform bacteria in which at least the endogenous accDA gene having the nucleic acid sequence comprising SEQ ID NO:
- 1 is over expressed, under conditions suitable for the production of the accDA gene product;
- b) accumulating the desired L-amino acid in the medium or in the cells of bacteria;
- and
- c) isolating the L-amino acid(s);
- and wherein said bacteria produce said L-amino acid(s).
A process for the production of L-amino acids selected from the group consisting of L-lysine, L-aspartic acid, L-asparagine, L-homoserine, L-threonine, L-isoleucine, and L-methionine comprising:
- a) culturing recombinant coryneform bacteria in which at least the endogenous accDA gene comprises a nucleic acid sequence encoding an amino acid sequence as set forth in SEQ ID NO:
- 3 is over expressed conditions suitable for the production of the accDA gene product;
- b) accumulating the desired L-amino acid in the medium or in the cells of bacteria;
- and
- c) isolating the L-amino acid(s);
- and wherein said bacteria produce said L-amino acid(s).
A process for the production of L-amino acid comprising:
- culturing recombinant bacteria under conditions suitable for overexpression of the accDA gene comprising a nucleic acid sequence encoding an amino acid sequence as set forth in SEQ ID NO:
- 3 and wherein said bacteria produce said L-amino acid selected from the group consisting of L-lysine, L-aspartic acid, L-asparagine, L-homoserine, L-threonine, L-isoleucine, and L-methionine.
Dependencies are inferred from the claim text.