Method for fluorescence microscopy, and fluorescence microscope
AssigneeLeica Microsystems Heidelberg GmbH
InventorsJohann Engelhardt, Juergen Hoffmann
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Abstract
The invention discloses a fluorescence microscope comprising a light source that emits excitation light for illumination of a specimen, means for defining a two-dimensional search region for the excitation and detection wavelengths, means for selecting a subregion from the search region, at least one detector that detects detected light proceeding from the specimen, and a display for displaying an image of at least a portion of the specimen. Furthermore the invention discloses a method for fluorescence microscopy.
Claims23
A method for fluorescence microscopy, wherein a specimen is illuminated with excitation light, wherein detected light proceeding from the specimen is detected, and wherein an image of at least a portion of the specimen is generated, comprising the steps of:
- defining a two-dimensional search region for excitation and detection wavelengths;
- determining, from the image of the specimen, quality features for subregions of the search region, the quality features being at least one of a brightness, a contrast, a resolution, a sharpness and a lifetime of the image;
- selecting a subregion on the basis of the quality features that have been determined;
- illuminating the specimen with excitation light of the excitation wavelengths of the selected subregion;
- and
- detecting the detected light of the detection wavelengths of the selected subregion.
A fluorescence microscope comprising:
- a light source that emits excitation light for illumination of a specimen,
- means for defining a two-dimensional search region for the excitation and detection wavelengths,
- means for selecting a subregion from the search region based on quality features determined from the image of the specimen, the quality features being at least one of a brightness, a contrast, a resolution, a sharpness and a lifetime of the image,
- at least one detector that detects detected light proceeding from the specimen, and
- a display for displaying an image of at least a portion of the specimen.
Dependencies are inferred from the claim text.