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US 6,806,399 B1Granted
Pollen-mediated method for transformation of maize, tomato or melon
AssigneeCarmel-Haifa University Economic Corp. Ltd.
InventorsAbraham Korol, Tzion Fahima, Eviator Nevo
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Abstract
A genotype-independent method for efficiently carrying out pollen-mediated transformation of maize, tomato or melon is described. The method uses pollen pretreated with silicon carbide and DNA to produce transformed plants with high efficiency and reproducibility.
Claims26
A method for genetic transformation of tomato or melon, said method comprising the steps of:
- (a) preparing a silicon carbide fibers solution;
- (b) preparing a pollen germination medium;
- (c) preparing a DNA solution;
- (d) mixing said silicon carbide fiber solution with said pollen germination medium and said DNA solution to form a mixture;
- (e) adding fresh pollen into said mixture to form a paste;
- (f) vortexing said paste for 30-60 seconds, thereby producing a vortexed paste;
- (g) applying said vortexed paste on female reproduction plant parts of melon or tomato plants for pollination;
- and
- (h) selecting transformants.
6 more elements
A method for genetic transformation of sexually reproducing maize, said method comprising the steps of:
- (a) preparing a silicon carbide fiber solution;
- (b) preparing a pollen germination medium;
- (c) preparing a DNA solution;
- (d) mixing said silicon carbide fiber solution with said pollen germination medium and said DNA solution to form a mixture;
- (e) adding fresh pollen into said mixture to form a paste;
- (f) vortexing said paste for 30 to 60 seconds, thereby producing a vortexed paste;
- (g) applying said vortexed paste on silks of maize plants for pollination;
- and
- (h) selecting transformants.
6 more elements
Dependencies are inferred from the claim text.