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US 6,806,362 B2Granted
pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
AssigneePromega Corporation
InventorsCraig E. Smith, Diana L. Holmes, Daniel J. Simpson, Jehoshua Katzenhendler, Rex M. Bitner, Josephine C. Grosch
Life of this patent
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Abstract
Disclosed are pH dependent ion exchange matrices, methods of making, and methods of use.
Claims25
A pH dependent ion exchange matrix, comprising:
- a solid support, and
- a plurality of ion exchange ligands, each first ion exchange ligand comprising:
- a cap comprising an amine with a pK of less than about 9;
- a spacer covalently attached to the cap, the spacer comprising a spacer alkyl chain with an amine terminus and an acidic moiety covalently attached to the spacer alkyl chain, wherein the acidic moiety is a carboxyl residue;
- and
- a linker comprising a linker alkyl chain covalently attached to the solid support at a first end of the linker alkyl chain and covalently attached to the amine terminus of the spacer at a second end of the linker alkyl chain;
- wherein the matrix has a capacity to adsorb to a target nucleic acid at a first pH, and to release the target nucleic acid at a desorption pH which is higher than the first pH.
4 more elements
A pH dependent ion exchange matrix, comprising:
- a solid support, and
- a plurality of ion exchange ligands, each first ion exchange ligand comprising:
- a cap comprising an amine with a pK of less than about 9;
- a spacer covalently attached to the cap, the spacer comprising a spacer alkyl chain with an amine terminus and an acidic moiety covalently attached to the spacer alkyl chain;
- and
- a linker comprising a linker alkyl chain covalently attached to the solid support at a first end of the linker alkyl chain and covalently attached to the amine terminus of the spacer at a second end of the linker alkyl chain;
- wherein the matrix has a capacity to adsorb to a target nucleic acid at a first pH, and to release the target nucleic acid at a desorption pH which is higher than the first pH, and wherein the cap further comprises an aromatic hydrocarbon ring.
4 more elements
A pH dependent ion exchange matrix, comprising:
- a solid support, and
- a plurality of ion exchange ligands, each first ion exchange ligand comprising:
- a cap comprising an amine with a pK of less than about 9;
- a spacer covalently attached to the cap, the spacer comprising a spacer alkyl chain with an amine terminus and an acidic moiety covalently attached to the spacer alkyl chain;
- and
- a linker comprising a linker alkyl chain covalently attached to the solid support at a first end of the linker alkyl chain and covalently attached to the amine terminus of the spacer at a second end of the linker alkyl chain;
- wherein the matrix has a capacity to adsorb to a target nucleic acid at a first pH, and to release the target nucleic acid at a desorption pH which is higher than the first pH, and wherein the aromatic hydrocarbon covalently liked to the spacer defines a basic amino acid moiety selected from the group consisting of histidine and histamine.
4 more elements
Dependencies are inferred from the claim text.