Causative agent of the mystery swine disease, vaccine compositions and diagnostic kits
AssigneeStichting Centraal Diergeneeskundig Instituut
InventorsGert Wensvoort, Catharinus Terpstra, Joannes Maria Anthonis Pol, Robertus Jacobus Maria Moormann, Johanna Jacoba Maria Meulenberg
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Abstract
Composition of matter comprising the causative agent of Mystery Swine Disease, Lelystad Agent, in a live, attenuated, dead, or recombinant form, or a part or component of it. Vaccine compositions and diagnostic kits based thereon. Recombinant nucleic acid comprising a Lelystad Agent-specific nucleotide sequence. Peptides comprising a Lelystad Agent-specific amino acid sequence. Lelystad Agent-specific antibodies.
Claims3
A diagnostic kit for detecting an antibody which specifically recognizes Lelystad Agent in a biological sample, said diagnostic kit comprising
- an antigen selected from the group consisting of virus, protein, polypeptide, and peptide, said antigen immunoreactive with serum antibodies of a sow, said serum antibodies obtained by:
- a) intranasally inoculating a specific pathogen free sow with two milliliters of a virus essentially corresponding to the virus identified as deposit number I-1102, deposited Jun. 5, 1991 with the Institut Pasteur, Paris, France (at passage level 3, titer 104.8 TCID50/milliliter);
- and further comprising
- b) collecting serum antibodies from the thus inoculated sow after 25 to 33 days, and
- means for detecting an antibody.
A diagnostic kit for detecting an antibody which specifically recognizes Lelystad Agent in a biological sample said Lelystad Agent essentially corresponding to the isolated Lelystad Agent deposited 5 Jun. 1991 with the Institut Pasteur, Paris, France, deposit number I-1102, said diagnostic kit comprising:
- a protein or antigenic polypeptide from Lelystad Agent, and
- means for detecting an antibody reacting with said protein or antigenic polypeptide.
Dependencies are inferred from the claim text.