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US 6,806,064 B2Granted
Baculovirus enhancer-like sequence
AssigneeAcademia Sinica
InventorYu-Chan Chao
Life of this patent
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Abstract
The invention features nucleic acid constructs and related methods useful for the high level expression of a heterologous protein in a cell, e.g., an insect cell. The constructs include an enhancer-like sequence located upstream from the polyhedrin gene locus in the genome of baculovirus, which sequence enhances the activity of an operably linked promoter.
Claims33
A nucleic acid construct, comprising:
- a nucleotide sequence of less than 10,000 nucleotides, said nucleotide sequence including SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, wherein a 5′ or 3′ sequence immediately flanking said nucleotide sequence is different from the 5′ or 3′ sequence immediately flanking said nucleotide sequence in a AcMNPV genome;
- and
- a promoter operably linked to a heterologous coding sequence,
- wherein said nucleotide sequence is operably linked to said promoter.
1 more element
A method of producing a polypeptide, comprising:
- providing a nucleic acid construct including a nucleotide sequence of less than 10,000 nucleotides, said nucleotide sequence including SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, wherein a 5′ or 3′ sequence immediately flanking said nucleotide sequence is different from the 5′ or 3′ sequence immediately flanking said nucleotide sequence in a AcMNPV genome, wherein said nucleotide sequence is operably linked to a promoter that is operably linked to a coding sequence;
- introducing said nucleic acid construct into a cell;
- and
- allowing said cell to express a polypeptide encoded by said coding sequence, thereby producing a polypeptide.
1 more element
Dependencies are inferred from the claim text.