Fungal transcriptional activator useful in methods for producing polypeptides
AssigneeNovozymes A/S
InventorsCarsten M. Hjort, Cees van den Hondel, Peter J. Punt, Frank H. J. Schuren, Tove Christensen
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Abstract
The present invention relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The invention also relates to nucleic acid constructs, vectors and host cells comprising the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.
Claims32
An isolated nucleic acid sequence, which comprises a sequence encoding a polypeptide that has transcriptional activation activity, wherein the sequence is:
- (a) a nucleic acid sequence which hybridizes under high stringency conditions with (i) the nucleic acid sequence of SEQ ID NO:
- 1 or (ii) its complementary strand, wherein the high stringency conditions are defined by prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing at 65° C. for 30 minutes in 2×SSC and 0.2% SDS;
- or
- (b) a nucleic acid sequence that encodes a fragment of SEQ ID NO:
- 2, wherein the fragment has transcriptional activation activity.
Dependencies are inferred from the claim text.