Maize cellulose synthases and uses thereof
AssigneePioneer Hi-Bred Int'l., Inc.
InventorsKanwarpal S. Dhugga, Timothy G. Helentjaris, Benjamin A. Bowen, Xun Wang
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Abstract
The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
Claims16
An isolated polynucleotide encoding a functional cellulose synthase, said polynucleotide selected from the group consisting of:
- a) a polynucleotide having at least 90% sequence identity, as determined by the BLAST 2.0 algorithm under default parameters, to the full length nucleotide sequence of SEQ ID NO:9.
- b) a polynucleotide encoding the polypeptide of SEQ ID NO:
- 10;
- and
- c) the polynucleotide of SEQ ID NO:
- 9.
A method of modulating the level of cellulose synthase in a plant cell, comprising:
- transforming the plant cell with a recombinant expression cassette comprising a polynucleotide encoding a functional cellulose synthase operably linked to a promoter;
- wherein the polynucleotide encodes a polypeptide selected from the group consisting of:
- the polypeptide of SEQ ID NO:
- 10;
- and a polypeptide having at least 90% sequence identity to the full length amino acid sequence of SEQ ID NO:
- 10, wherein said sequence identity is determined with BLAST 2.0 under default parameters;
- and
- culturing the transformed plant cell under plant cell growing conditions;
- for a time sufficient to modulate wherein the level of cellulose synthase in said transformed plant cell is modulated.
A transgenic plant comprising a recombinant expression cassette comprising a polynucleotide encoding a polypeptide having cellulose synthase activity operably linked, in sense or antisense orientation, to a promoter, wherein said polypeptide is selected from the group consisting of:
- the polypeptide of SEQ ID NO:
- 10;
- and a polypeptide having at least 90% sequence identity to the full length amino acid sequence of SEQ ID NO:
- 10, wherein said sequence identity is determined with BLAST 2.0 under default parameters.
Dependencies are inferred from the claim text.