Antibodies associated with alterations in bone density
AssigneeDarwin Discovery, Ltd.
InventorsMary E. Brunkow, David J. Galas, Brian Kovacevich, John T. Mulligan, Bryan W. Paeper, Jeffrey Van Ness, David G. Winkler
Life of this patent
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Abstract
Compositions and methods for increasing bone density using antibodies directed to a novel class or family of TGF-β binding proteins are provided. The disclosed compositions are useful, for example, in the diagnosis, prevention and/or treatment of diseases associated with a loss of bone density, for example osteoporosis.
Claims12
An isolate antibody or antigen binding fragment thereof which specifically binds to a TGF-beta binding protein, wherein said binding protein is selected from the group consisting of:
- (a) a polypeptide encoded by a polynucleotide that comprises a nucleotide sequence selected from SEQ ID NOs:1, 5, 9, 11, 13, and 15, or a complementary sequence thereto which encodes a TGF-beta birding protein, and
- (b) a polypeptide that comprises an amino acid sequence selected from SEQ ID NOs:
- 2, 6, 10, 12, 14, and 16.
An isolated antibody or antigen binding fragment thereof which specifically binds to a TGF-beta binding protein, wherein said binding protein is selected from the group consisting of:
- (a) a polypeptide encoded by a polynucleotide that comprises a nucleotide sequence selected from SEQ ID NOs:1, 5, 7, 9, 11, 13, and 15, or a complementary sequence thereto which encodes a TGF-beta binding protein;
- and
- (b) a polypeptide that comprises an amino acid sequence selected from SEQ ID NOs:
- 2, 6, 3, 10, 12, 14, and 16.
A method of producing monoclonal antibodies, comprising:
- (a) immunizing an animal with a TGF-beta binding protein or portion thereof, wherein said binding protein is selected from the group consisting of:
- (i) a polypeptide encoded by a polynucleotide that comprises a nucleotide sequence selected from SEQ ID NOs:1, 5, 7, 9, 11, 13, and 15, or a complementary sequence thereto which encodes a TGF-beta binding protein, and
- (ii) a polypeptide that comprises an amino acid sequence selected from SEQ ID NOs:
- 2, 6, 8, 10, 12, 14, and 16;
- (b) harvesting spleen cells from said animal;
- (c) fusing said spleen cells with a myeloma cell line;
- and
- (d) culturing said fused cells under conditions that allow the production of said antibody.
A method for immunizing an animal comprising injecting an animal with a TGF-beta binding protein or portion thereof, wherein said binding protein is selected from the group consisting of:
- (i) a polypeptide encoded by a polynucleotide that comprises a nucleotide sequence selected from SEQ ID NOs:1, 5, 7, 9, 11, 13, and 15, or a complementary sequence thereto, and
- (ii) a polypeptide that comprises an amino acid sequence selected from SEQ ID NOs:
- 2, 6, 8, 10, 12, 14, and 16.
Dependencies are inferred from the claim text.