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US 6,800,492 B2Granted
Chimeric GFP-aequorin as bioluminescent Ca++ reporters at the single cell level
AssigneeInstitute Pasteur
InventorsValerie Baubet, Hervé LeMouellic, Philippe Brulet
Life of this patent
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Abstract
A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer (CRET).
Claims12
A purified polypeptide having the amino acid sequence of SEQ ID NO:
- 5.
A peptide linker comprising the amino acid sequence of SEQ ID NO:
- 22.
A fusion protein of the formula:
- GFP-LINKER-AEQ:
- wherein GFP is green fluorescent protein;
- AEQ is aequorin;
- and
- LINKER is a polypeptide of 14-50 amino acids.
2 more elements
Dependencies are inferred from the claim text.