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AssigneeGeron Corporation
InventorsAndrea G. Bodnar, Choy-Pik Chiu, Joseph D. Gold, Margaret Inokuma, James T. Murai, Michael D. West
Estimating the term…
Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.
A cellular composition comprising undifferentiated primate primordial stem (pPS) cells proliferating on an extracellular matrix, wherein the composition is free of feeder cells.
A composition comprising:
A cellular composition comprising undifferentiated pPS cells proliterating on an extracellular matrix, obtained by passaging pPS cells cultured on feeder cells to a new growth environment having an extracellular matrix but free of feeder cells;
A cell population consisting essentially of primate embryonic stem (ES) cells proliferating in culture on an extracellular matrix in a manner such that at least 50% of the proliferating ES cells are undifferentiated.
A cellular composition comprising undifferentiated human embryonic stem cells proliferating on an extracellular matrix, wherein the composition is free of feeder cells.
A cellular composition comprising undifferentiated human embryonic stem (ES) cells proliferating on an extracellular matrix, obtained by passaging human ES cells cultured on feeder cells to a new growth environment having an extracellular matrix but free of feeder cells;
Dependencies are inferred from the claim text.