D-hydantoinase from Ochrobactrum anthropi
AssigneeBristol-Myers Squibb Company
InventorsMichael Politino, Sean M. Tonzi, Guna Romancik, John J. Usher, David A. Lowe
Life of this patent
Estimating the term…
Abstract
The present invention relates to a novel D-hydantoinase from Ochrobactrum anthropi that enantio-selectively hydrolyzes D-hydantoins to their corresponding D-N-carbamoyl-amino acids; nucleic acids that encode for the enzyme; expression vectors including the nucleic acids; and host cells capable of expressing the enzyme.
Claims22
An isolated nucleic acid molecule comprising a sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated nucleic acid molecule having a sequence that is complementary to a nucleic acid molecule that encodes SEQ ID NO:2.
An isolated nucleic acid molecule capable of hybridizing under stringent conditions to SEQ ID NO:1.
An isolated nucleic acid molecule encoding a polypeptide having an amino acid sequence that is at least 80% homologous to SEQ ID NO:2.
An isolated polypeptide having an amino acid sequence that is at least 80% homologous to SEQ ID NO:2.
An isolated polypeptide having the amino acid sequence of SEQ ID NO:2.
A process for stereoselectively converting a 5-monosubstituted hydantoin comprising contacting said 5-monosubstituted hydantoin with the D-hydantoinase as shown in SEQ ID NO:2, or a biologically active fragment or analog thereof, under conditions suitable to form the corresponding D-N-carbamoyl-amino acid.
Dependencies are inferred from the claim text.