Recombinant, replication defective CELO virus and CELO virus DNA
AssigneeBoehringer Ingelheim International GmbH
InventorsMatthew Cotten, Jolanta Glotzer, Anne-Isabelle Michou
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Abstract
Recombinant CELO virus or CELO virus DNA with a deletion or disruption of the Gam1 gene, optionally combined with deletions at the right or left end of the viral genome that allow insertion of large pieces of foreign DNA. The virus is useful as a vaccine for animals, in particular birds, and for gene therapy and vaccine applications in humans. The virus can also be used for recombinant protein production.
Claims30
A recombinant CELO virus or CELO virus DNA, characterized in that the virus or DNA comprises a CELO wild type genome, wherein the genome contains at least one deletion, said deletion consisting of nucleotides 37,391-37,972 of the CELO wild type virus genome, wherein said deletion results in a complete loss of Gam1 expression or prevents the expression of a functional Gam1 protein, wherein the genome can be complimented by heat shock or heat shock protein 40.
A recombinant CELO virus or CELO virus DNA, characterized in that the virus or DNA comprises a CELO wild type genome, wherein the genome contains at least one deletion, said deletion consisting of nucleotides 36,818-37,972 of the CELO wild type virus genome, wherein said deletion results in a complete loss of Gam1 expression or prevents the expression of a functional Gam1 protein, wherein the genome can be complimented by heat shock or heat shock protein 40.
Dependencies are inferred from the claim text.