US 6,794,167 B2 · Modified naphthalene dioxygenases and methods of use

Abstract

The invention provides an NDO or NDO related complex comprising at least one alpha-subunit polypeptide that comprises: 1) a substituted amino acid at the position corresponding to position 352 in NDO, 2) a substituted amino acid at the position corresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO, or 3) a substituted amino acid at the position corresponding to position 352 in NDO, and a substituted amino acid at the position corresponding to position 201, 202, 260, 316, 351, 358, 362, or 366 in NDO; or a catalytically active fragment thereof. The invention also provides DNA encoding such polypeptides, host cells augmented by such DNA, and methods for using the enzymes or host cells to provide useful and novel synthons. The invention also provides novel compounds prepared with the complexes or methods of the invention.

Claims15

Dependent claims

A dioxygenase complex comprising a plurality of polypeptides, wherein the complex catalyzes the oxidation of an aromatic substrate and comprises at least one alpha-subunit polypeptide selected from the group consisting of:

(a) a polypeptide comprising SEQ ID NO:
26 with a substituted amino acid at position 352 of SEQ ID NO:
26,
(b) a polypeptide comprising SEQ ID NO:
26 with a substituted amino acid at position 201, 202, 260, 316, 351, 358, 362, or 366 of SEQ ID NO:
26,
(c) a polypeptide comprising SEQ ID NO:
26 with a substituted amino acid at position 352 of SEQ ID NO:
26 and a substituted amino acid at position 201, 202, 260, 316, 351, 358, 362, or 366 of SEQ ID NO:
26,
or a fragment of said dioxygenase complex that catalyzes oxidation of an aromatic substrate.

8 more elements

Dependencies are inferred from the claim text.

Modified naphthalene dioxygenases and methods of use

Life of this patent

Modified naphthalene dioxygenases and methods of use

Life of this patent

Abstract

Claims15