US 6,794,137 B2 · Gene markers useful for detecting skin damage in response to ultraviolet radiation

Abstract

The cellular response to ultraviolet radiation exposure has been characterized on the molecular level through the use of high density gene array technology. Nucleic acid molecules and protein molecules, the expression of which are repressed or induced in response to ultraviolet radiation exposure, are identified according to a temporal pattern of altered expression post ultraviolet radiation exposure. Methods are disclosed that utilized these ultraviolet radiation-regulated molecules as markers for ultraviolet radiation exposure. Other screening methods of the invention are designed for the identification of compounds that modulate the response of a cell to ultraviolet radiation exposure. The invention also provides compositions useful for drug screening or pharmaceutical purposes.

Claims92

Dependent claims

A method for detecting exposure of a cell to ultraviolet radiation, comprising measuring the levels of a plurality of RNA molecules in the cell for at least one time point after ultraviolet radiation exposure to establish a pattern of expression, the response of the cell to ultraviolet radiation exposure comprising at least one of the following:

(a) a first response comprising altered expression of at least one nucleic acid molecule encoding a transcription factor protein, at least one nucleic acid molecule encoding a signal transducing protein, and at least one nucleic acid molecule encoding a mitochondrial protein;
(b) a second response comprising altered expression of at least one nucleic acid molecule encoding a secreted growth factor, at least one nucleic acid molecule encoding a cytokine, and at least one nucleic acid molecule encoding a chemokine;
and
(c) a third response comprising altered expression of at least one nucleic acid molecule encoding an actin-binding protein, at least one nucleic acid molecule encoding a desmosomal protein, and at least one nucleic acid molecule encoding a tubulin protein;
and
wherein if the pattern of expression for the cell is substantially similar to the response of the cell to ultraviolet radiation, the cell was exposed to ultraviolet radiation.

3 more elements

A method for detecting exposure of a cell to ultraviolet radiation comprising:

(a) measuring the levels of a plurality of RNA molecules in the cell by expression array analysis, comprising:
(i) isolating RNA from the cell post-ultraviolet radiation exposure;
(ii) creating a test expression array through nucleic acid hybridization between a labeled probe complementary to the RNA and an expression array substrate;
(iii) analyzing the test expression array to create a test expression array data set;
and
(iv) comparing the test expression array data set to a control expression array data set;
and
(b) analyzing the levels of the plurality of RNA molecules to establish a pattern of expression for the cell, the response of the cell to ultraviolet radiation exposure comprising at least one of the following:
(i) a first response comprising altered expression of at least one nucleic acid molecule encoding a transcription factor protein, at least one nucleic acid molecule encoding a signal transducing protein, and at least one nucleic acid molecule encoding a mitochondrial protein;
(ii) a second response comprising altered expression of at least one nucleic acid molecule molecule encoding a secreted growth factor, at least one nucleic acid encoding a cytokine, and at least one nucleic acid encoding a chemokine;
and
(iii) a third response comprising altered expression of at least one nucleic acid molecule encoding an actin-binding protein, at least one nucleic acid molecule encoding a desmosomal protein, and at least one nucleic acid molecule encoding a tubulin protein,
wherein if the pattern of expression for the cell is substantially similar to the response of the cell to ultraviolet radiation, the cell was exposed to ultraviolet radiation.

10 more elements

A method to detect exposure of a cell to ultraviolet radiation comprising:

(a) measuring the levels of a plurality of RNA molecules in the cell by expression array analysis, comprising:
(i) isolating RNA from the cell post ultraviolet radiation exposure;
(ii) creating a test expression array through nucleic acid hybridization between a labeled probe complementary to the RNA and an expression array substrate;
(iii) analyzing the test expression array to create a test expression array data set;
and
(iv) comparing the test expression array data set to a control expression array data;
and
(b) analyzing the levels of the plurality of RNA molecules and thereby establishing a response pattern of the cell,
wherein exposure of the cell to ultraviolet radiation is indicated by the altered pattern of expression comprising the following:
(i) a first response comprising the altered pattern of expression of at least one polynucleotide selected from the group consisting of a nucleic acid molecule encoding a transcription factor protein, a nucleic acid molecule encoding a signal transducing protein, and a nucleic acid molecule encoding a mitochondrial protein;
(ii) a second response comprising the altered pattern of expression of at least one polynucleotide selected from the group consisting of a nucleic acid molecule encoding a secreted growth factor, a nucleic acid molecule encoding a cytokine, and a nucleic acid molecule encoding a chemokine;
and
(iii) a third response comprising the altered pattern of expression of at least one polynucleotide selected from the group consisting of a nucleic acid molecule encoding an actin-binding protein, a nucleic acid molecule encoding a desmosomal protein, and a nucleic acid molecule encoding a tubulin protein.

10 more elements

Dependencies are inferred from the claim text.

Gene markers useful for detecting skin damage in response to ultraviolet radiation

Life of this patent

Gene markers useful for detecting skin damage in response to ultraviolet radiation

Life of this patent

Abstract

Claims92