Nucleic acid molecule coding for beta-amylase, plants synthesizing a modified starch, method of production and applications
AssigneeBayer CropScience AG
InventorClaus Frohberg
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Abstract
The present invention relates to nucleic acid molecules coding for a protein with beta-amylase activity from potato and to a method for the production of transgenic plant cells and plants synthesizing a modified starch. The invention also relates to vectors and host cells containing the nucleic acid molecules according to the invention, the plant cells and plants produced using the method according to the invention and the starch synthesized by the plant cells and plants according to the invention. The invention also relates to a method of production of said starch.
Claims20
An isolated nucleic acid molecule encoding a protein with the function of a potato β-amylase, selected from the group consisting of:
- a) a nucleic acid molecule encoding the amino acid sequence of SEQ ID NO:
- 2;
- b) a nucleic acid molecule having the nucleotide sequence of SEQ ID NO:
- 1;
- c) a nucleic acid molecule which hybridizes with, or is complementary to, the nucleic acid molecules stated under a) or b), wherein hybridization is performed at a temperature of 68° C. in buffer comprising 2×SSC or 7% SDS and is followed by a wash at 68° C. in a wash buffer containing 0.2×SSC;
- and
- d) a nucleic acid molecule whose nucleotide sequence deviates from the sequence of the nucleic acid molecule stated under a)-c) owing to the degeneracy of the genetic code.
Dependencies are inferred from the claim text.