Assay for directly detecting a RS virus related biological cell in a body fluid sample
AssigneeBESST-TEST ApS
InventorsMichael Rud Lassen, Morten Breindahl
Life of this patent
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Abstract
The present invention relates to a kit and an assay method for rapidly and directly detecting a predetermined respiratory syncytial virus-related biological cell in a sample, even when present in an amount of less than about 2000 cells per microliter. In a preferred embodiment, a labeled antibody targets a nucleoprotein or a glycoprotein of RS virus. One may further detect an inflammatory indicator in the sample.
Claims70
A kit for directly detecting a respiratory syncytial (RS) virus infected cell or an RS virus biological particle present in a sample in an amount of less than about 2000 cells or particles per microlitre (10−6 liter), said kit comprising
- i) a solid support comprising an application zone for applying the sample and a separate detection zone, and
- ii) a conjugate comprising a polymeric carrier molecule bound to
- a) at least one targeting species capable of directly binding an RS virus infected cell or RS virus biological particle, and
- b) at least one labelling species,
- wherein the application zone and the detection zone can form a liquid contact, the application zone comprises at least one of said conjugate, said conjugate being movable, and the detection zone comprises at least one targeting species which is bound to the solid support and is capable directly of binding an RS virus infected cell or RS virus biological particle.
Dependencies are inferred from the claim text.