Mobile phase dilution scheme for enhanced chromatography
AssigneeWaters Corporation
InventorsThomas E. Wheat, Charles H. Phoebe, Mark K. Baynham, Uwe Dieter Neue, Raymond P. Fisk, Richard C. Turner
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Abstract
A method and device for increasing the loading capacity of a chromatography column through dilution of a mobile phase at the head of the column. A strong mobile phase is provided for dissolving a sample. A sample is injected into the strong mobile phase, and subsequently diluted with a weak mobile phase. The resulting sample-containing weak mobile phase is passed through a chromatography column for retention and separation of the components of the sample.
Claims21
An enhanced chromatographic method of separating a sample in a chromatography system, comprising:
- injecting a sample solution into a strong mobile phase to form a sample-containing strong mobile phase,
- diluting the sample-containing strong mobile phase to form a sample-containing weak mobile phase, and
- passing the sample-containing weak mobile phase through a chromatography column, wherein the pH of the sample-containing weak mobile phase is different from the pH of the sample-containing strong mobile phase, thereby providing an enhanced chromatographic method.
Dependencies are inferred from the claim text.