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US 6,788,409 B2Granted
Flow cell system for solubility testing
AssigneeBecton, Dickinson and Company
InventorJoseph J. Goodwin
Life of this patent
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AssigneeBecton, Dickinson and Company
InventorJoseph J. Goodwin
Estimating the term…
The invention provides a flow cell system for high throughput solubility testing of compounds that is particularly useful for screening large compound collections, such as combinatorial chemical libraries or other such synthetic chemical libraries. The system includes a flow cell to channel a fluid sheath which itself channels a continuous or intermittent sample liquid stream containing a compound for testing. Where the concentration of compound in the sample exceeds the solubility limit, particles of the test compound precipitate. These particles scatter the light from an interrogating light beam directed at the sample liquid stream. Individual flashes of scattered light are detected as electronic pulse signals from the detector and their intensity analyzed according to intensity and number of events. System software analyzes the stored pulse signal data in real-time to determine whether there is a significant increase in scattering over the highly variable background scattering. The software then outputs a signal to effectuate selection of a subsequent sample, which may be a higher concentration sample of the same compound or a sample of a subsequent compound for solubility testing. The system also automatically realigns the sample liquid flow after a preset number of samples or in the event that control particles or beads are not efficiently detected.
A flow cell system for solubility testing of a compound in a sample liquid, comprising:
A method for solubility testing of a compound in a sample liquid, comprising:
A method for solubility testing of a compound in a sample liquid, comprising:
A method for maintaining the sample stream alignment in a flow cell, comprising:
A processor for real-time processing of multiple electronic pulse detection signals from a flow cytometer adapted for solubility testing, wherein each of the signals is allocated to one of a series of channels, each channel detecting electronic pulse detection signals within a preset signal amplitude range, to provide multichannel sample data, and outputting the sample data for effectuating selection of a subsequent sample liquid from a plurality of subsequent sample liquids for solubility testing.
A processor for real-time processing of multiple electronic pulse detection signals from a flow cytometer adapted for solubility testing, wherein each of the signals is allocated to one of a series of channels, each channel detecting electronic pulse detection signals within a preset signal amplitude range to provide multichannel control substance data, and thereby determining whether the sample liquid stream is aligned or misaligned, and outputting an effectuator signal if the sample liquid stream is misaligned;
A flow cell system for solubility testing of a compound in a sample liquid, comprising:
Dependencies are inferred from the claim text.