US 6,787,368 B1Granted

Biosensor method for detecting analytes in a liquid

AssigneeHelix BioPharma Corporation

InventorsWah Y. Wong, Heman Chao, Donald Segal, Jerry McElroy

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Abstract

A diagnostic method and device for use in detecting or quantitating an analyte present in a liquid sample. The method includes reacting an analyte-containing sample with reagents capable of generating a first coil-forming peptide in solution form. This peptide is then contacted with a biosensor whose detection surface has surface-bound molecules of a second, oppositely charged coil-forming peptide, under conditions effective to form a stable α-helical coiled-coil heterodimer on the detection surface. The formation of the coiled-coil heterodimer produces a measurable change in biosensor signal, which is measured to detect the presence of or quantitate the amount of analyte in a sample. Also disclosed is a biosensor device for carrying out the reaction.

Claims10

Dependent claims

A method for detecting or quantitating an analyte present in a liquid sample, comprising

reacting the liquid sample with an analyte-reaction reagent,
by said reacting, generating a solution of a first coil-forming peptide having a selected charge for interacting with a second, oppositely charged coil-forming peptide to form a stable α-helical coiled-coil heterodimer,
contacting the first coil-forming peptide generated by said reaction with a biosensor having a detection surface with surface-bound molecules of said second, oppositely charged coil-forming peptide, under conditions effective to form a stable α-helical coiled-coil heterodimer on said detection surface, where binding of the coil-forming peptide to the immobilized coil-forming peptide measurably alters a signal generated by the biosensor,
measuring the signal generated by the biosensor to determine whether said coiled-coil heterodimer formation on said detector surface has occurred, and
correlating said generated signal to the presence of the analyte in the sample.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 09/517,597Filed 2 Mar 2000 · us provisional application
Priority: 2 Mar 1999
Granted: 7 Sep 2004
Term ends: Not estimatedThe source records don’t support an estimate.

Classification3 codes

G01N 33/54373
Apparatus specially adapted for solid-phase testing: involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
G01N 33/5438
Involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings: electrodes
G01N
Investigating or analysing materials by determining their chemical or physical properties