Cytochrome P450 oxygenases and their uses
AssigneeWashington State University Research Foundation
InventorsRodney B. Croteau, Anne Schoendorf, Stefan Jennewein
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Abstract
Oxygenase enzymes and the use of such enzymes to produce paclitaxel (Taxol™), related taxoids, as well as intermediates in the Taxol biosynthetic pathway are disclosed. Also disclosed are nucleic acid sequences encoding the oxygenase enzymes.
Claims14
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a protein comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 64;
- wherein the protein has taxoid oxygenase activity.
An isolated nucleic acid molecule that:
- (a) hybridizes under hybridization stringency conditions with a nucleic acid probe, the probe comprising a sequence as set forth in SEQ ID NO:
- 51;
- and
- (b) encodes a protein having taxoid oxygenase activity;
- wherein the hybridization stringency conditions comprise hybridization in 5×SSC at 65° C. for 16 hours, two first washes in 2×SSC at room temperature for 15 minutes each, and two second washes in 2×SSC at 60° C. for 20 minutes each.
An isolated nucleic acid molecule comprising a nucleic acid sequence having at least 95% sequence identity with a nucleic acid sequence according to SEQ ID NO:
- 51;
- wherein the isolated nucleic acid molecule encodes a protein having taxoid oxygenase activity.
Dependencies are inferred from the claim text.