Recording of DNA sequences to enable them to be expressed in yeasts, and the transformed yeasts obtained

A process for transforming a substrate by enzymatic catalysis with an enzyme which is expressed in a yeast comprising:

• a) culturing, in the presence of the substrate to be transformed, a transformed yeast that expresses a protein of interest, said transformed yeast comprising a chimeric gene, said chimeric gene comprising a recombinant non-yeast DNA encoding a plant protein of interest, wherein an unmodified DNA corresponding to said recombinant non-yeast DNA contains a region having a high content of codons that are poorly suited for expression in yeasts, wherein a number of the codons that are poorly suited to yeasts are replaced in said region of said recombinant non-yeast DNA with synonymous codons coding for the same amino acid that are well-suited to yeasts, and wherein the number of replaced codons is sufficient to permit expression of the non-yeast DNA in yeasts, said chimeric gene being operable linked to heterologous 5′ and 3′ regulators elements which are able to function in a yeast;
• and
• b) recovering the transformed substrate from the yeast culture.

A recombinant non-yeast DNA encoding a plant protein of interest, wherein an unmodified DNA corresponding to said recombinant non-yeast DNA contains a region having a high content of codons that are poorly suited for expression in yeasts, wherein a number of the codons in the 5′ region of the DNA that are poorly suited for expression in yeasts are replaced in said 5′ region of said recombinant non-yeast DNA with synonymous codons coding for the same amino acid that are well-suited for expression in yeasts, wherein the number of replaced codons is sufficient to permit expression in yeasts, and wherein replaced codons are only in the 5′ region.

A recombinant non-yeast DNA encoding a plant cytochrome P450, in which the non-yeast DNA encoding the plant cytochrome, P450 contains a region having a high content of codons that are poorly suited for expression in yeasts, wherein a number of the codons that are poorly suited for expression in yeasts are replaced in said region of said recombinant non-yeast DNA with synonymous codons coding for the same amino acid that are well-suited for expression in yeasts, such that the number of replaced codons is sufficient to permit expression of the plant cytochrome P450 DNA in yeasts.