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AssigneeDegussa AG
InventorsFrank Elischweski, Jorn Kalinowski, Alfred Puhler, Nicole Dusch, Jurgen Dohmen, Mike Farwick, Georg Thierbach
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The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.
A process for the preparation and improvement of a pantothenic acid-producing microorganism comprising amplifying the endogenous panE gene in said microorganism and then incubating said microorganism under conditions suitable for the production of the panE gene product, ketopantoate reductase, wherein the microorganism is selected from the group consisting of Escherichia and Saccharomyces.
The plasmid vector pFE80 characterized by the restriction map shown in FIG. 6 and deposited as E. coli K12 strain MG 1655/pFE80 under deposit number DSM 12414.
The plasmid vector pFE65, characterized by the restriction map shown in FIG. 5 and deposited as E. coli K12 strain MG 1655/pFE65 under deposit number DSM 12382.
The plasmid vector pFE32, characterized by the restriction map shown in FIG. 4 and deposited as E. coli K12 strain MG 1655/pFE32 under the deposit number DSM 12413.
Dependencies are inferred from the claim text.