Back to results
US 6,787,326 B1Granted
Interaction between the VHL tumor suppressor and hypoxia inducible factor, and assay methods relating thereto
AssigneeIsis Innovation Limited
InventorsPeter John Ratcliffe, Patrick Henry Maxwell, Christopher William Pugh
Life of this patent
Estimating the term…
Abstract
The invention relates to the finding that the VHL tumour suppressor protein regulates hypoxia inducible factor α subunits, by targeting HIF α for destruction in normoxic, but not hypoxic cells. The invention provides assays for modulators of this interaction, and peptides based upon HIF α subunit sequence which may modulate this interaction.
Claims16
An assay for a modulator of VHL-HIF α subunit interaction, which comprises:
- a) bringing into contact a VHL protein, an HIF α subunit protein and a putative modulator compound under conditions where the VHL protein and the HIF α subunit protein, in the absence of modulator, are capable of forming a complex;
- and
- b) measuring the degree of inhibition of complex formation caused by said modulator compound.
An assay for a modulator which promotes VHL-HIF α subunit interaction, which assay comprises:
- a) bringing into contact an HIF α subunit protein and a putative modulator compound in the presence or absence of a VHL protein.
- b) providing a VHL protein where said protein is absent in step (a);
- and
- c) determining whether the VHL-HIF α subunit interaction has been promoted by the presence of the modulator.
1 more element
Dependencies are inferred from the claim text.