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AssigneesIatron Laboratories, Inc., Kikkoman Corporation
InventorsKazuyuki Sugiyama, Nobuhiro Hoshino, Hiroki Tatsumi, Satoshi Fukuda
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Provided are novel complexes containing a crosslinked avidin, an analyzing method and analyzing reagents and kits whereby a compound to be analyzed can be quickly, conveniently and accurately analyzed while taking advantage of the avidin-biotin reaction. The complexes contain at least two homogeneous or heterogeneous biotin-introduced products and one crosslinked avidin sandwiched therebetween. In the analyzing method, the homogeneous or heterogeneous biotin-introduced products and the crosslinked avidin are used. The analyzing reagent contains the crosslinked avidin. The analyzing kit contains the crosslinked avidin and a biotinylating agent.
A biotin-avidin-biotin complex comprising at least two biotinylated substances which are the same or different, and a crosslinked avidin sandwiched therebetween, wherein said crosslinked avidin consists of an avidin monomer or an avidin polymer having intermolecular crosslinkages between two or more avidin monomers, wherein each of said avidin monomers has intramolecular crosslinkages between avidin subunits, and wherein only the avidin portion of the complex is crosslinked.
A method for analyzing a compound to be analyzed, said method comprising the steps of:
An analyzing reagent comprising a mixture of:
A method for analyzing a compound to be analyzed, said method comprising the steps of:
Dependencies are inferred from the claim text.