In vitro methods for determining in vivo thrombotic events

A method of detecting a C-terminal heavy chain platelet glycoprotein receptor (hGPIIb) polypeptide in a body fluid sample, comprising the steps of:

• a. admixing an aliquot of the body fluid sample with a composition including an antibody to form an immunoreaction admixture, wherein the antibody:
• i. immunoreacts with the C-terminal hGPIIb polypeptide including an amino acid sequence of PQPPVNPLKVDWGLPIPSPSP (SEQ ID NO:3 from residue 173 to residue 193), PQPPVNPLK (SEQ ID NO:3 from residue 173 to residue 181), PLKVDWGLPIP (SEQ ID NO:3 from residue 179 to residue 189), PIPSPSPIHPAHHK (SEQ ID NO:3 from residue 187 to residue 200), or IHPAHHK (SEQ ID NO:3 from residue 194 to residue 200), and
• ii. does not specifically immunoreact with an amino acid sequence represented by the formula REQNSLDSWGPK (SEQ ID NO:3 from residue 113 to residue 124);
• b. maintaining the immunoreaction admixture under biological assay conditions for a time period sufficient to form an immunoreaction product;
• and
• c. detecting the immunoreaction product, and thereby the C-terminal hGPIIb polypeptide.