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US 6,787,314 B2Granted
Polynucleotide separation method and apparatus therefor
AssigneeHitachi, Ltd.
InventorsKenji Yasuda, Kazunori Okano, Hirokazu Kato
Life of this patent
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Abstract
Different probes each having a specific base sequence are immobilized to each of independent areas formed on the surface of a substrate, complementary polynucleotides in a sample solution are hybridized to the probes, and each of the independent areas on the substrate is heated and then cooled in sequence, and hence the solution is recovered to extract different polynucleotides separately corresponding to individual probes.
Claims16
A cell components recovering apparatus comprising:
- a substrate being disposed in a separation cell, wherein sample solution containing cells is supplied on a surface of the substrate, the substrate having a plurality of independent areas formed on its surface;
- capturing means for capturing each of the cells one by one separately on each of the plurality of independent areas;
- and
- temperature control means for heating the surface of the substrate at one area of the plurality of independent areas to a predetermined temperature to destroy the cell captured at said one area to liberate cell components from the cell captured at said one area.
1 more element
A cell components recovering apparatus comprising:
- a substrate being disposed in a separation cell, wherein sample solution containing cells is supplied on a surface of the substrate, the substrate having a plurality of independent areas formed on its surface;
- capturing means for capturing each of the cells one by one separately on each of the plurality of independent areas;
- means for identifying a position of one area of said plurality of areas where a cell to be destroyed is present;
- and
- temperature control means for heating the surface of the substrate at said one area to a predetermined temperature to destroy the cell captured therein to liberate cell components from the cell captured therein into the separation cell.
2 more elements
Dependencies are inferred from the claim text.