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US 6,787,312 B2Granted
Treatment of substrates for immobilizing biomolecules
AssigneeCorning Incorporated
InventorsYijia Bao, Guangshan Li, Nalini Raghavachari, Mingqian He, Xinying Xie, Yuming Xie
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Abstract
A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. The method includes contacting at least a portion of a substrate with a reducing agent such as a hydride. Treatment with an appropriate reducing agent substantially eliminates autofluorescence on substrates.
Claims15
A method for reducing auto-fluorescence on a substrate, the method comprising:
- providing a substrate with at least a first surface, said first surface being either with or without either a biological or a synthetic molecule immobilized thereon, or having either a coated or uncoated surface with a residual fluorescence;
- and
- treating at least a portion of the first surface of the substrate with a reducing agent for longer than about 5 minutes to reduce auto-fluorescence on the substrate, wherein said autofluorescence is reduced by at least an order of magnitude RFU.
The method of 5 wherein the aqueous solution contains between 0.2% and 0.3% sodium borohydride by volume.
Dependencies are inferred from the claim text.