Lysate clearance and nucleic acid isolation using silanized silica matrices

The method of isolating a target nucleic acid from a nucleic acid adsorption solution, comprising the steps of:

• (a) providing a silanized silica matrix comprising a silica solid phase with a plurality of silane ligands covalently attached thereto, wherein each of the plurality of silane ligands is of the general formula:
• wherein R1 and R2 are each a subunit selected from the group consisting of a hydrocarbon chain having from 1 to 5 carbon atoms, an alkoxy having from 1 to 5 carbon atoms, a halogen atom, a hydrogen atom, a hydroxy, an alkyl chain having from 4 to 10 carbon atoms interrupted by an oxy residue wherein up to five of the carbon atoms is substituted by a group selected from the group consisting of a halogen, an alkoxy having from 1 to 3 carbon atoms, a cyano having from 1 to 3 carbon atoms, and a hydroxy;
• wherein R3 is a hydrocarbon chain having from 1 to 20 carbon atoms substituted by at least one hydroxy, an alkyl chain having from 4 to 20 carbon atoms interrupted by at least one oxy group wherein up to ten carbon atoms are replaced by a moiety selected from the group consisting of a halogen, a cyano having from 1 to 3 carbon atoms, an alkoxy having from 1 to 3 carbon atoms, a hydroxyl, an epoxy, and a linkage to another silane ligand;
• (b) combining the silanized silica matrix with a nucleic acid adsorption solution having a pH of up to about pH 8.0, the nucleic acid adsorption solution comprising the target nucleic acid and at least one non-target material, wherein the target nucleic acid selectively adsorbs to the silanized silica matrix, thereby forming a complex;
• and
• (c) separating the complex from the nucleic acid adsorption solution.

A method of isolating a target nucleic acid from a nucleic acid adsorption solution using a silanized magnetic particle, comprising the steps of:

• (a) providing a silanized silica magnetic particle, comprising a silica magnetic particle with a plurality of silane ligands covalently attached thereto, wherein each of the plurality of silane ligands is of the general formula:
• wherein formula, R1 and R2 are each independently selected from the group consisting of —OH, —CH3, —OCH3, or —OCH2CH3 and a linkage to another silane ligand to generate a higher order polymer;
• (b) combining the silanized silica magnetic particle with a nucleic acid adsorption solution having a pH of up to about pH 8.0, the nucleic acid adsorption solution comprising the target nucleic acid and at least one non-target material, wherein the target nucleic acid selectively adsorbs to the silanized silica magnetic particle, thereby forming a complex;
• and
• (c) separating the complex from the adsorption solution.