Loading patent…
AssigneeThe Rockefeller University
InventorsTakashi Aoyama, Jianru Zuo, Nam-Hai Chua
Estimating the term…
A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.
A vector comprising a DNA sequence encoding a transcription factor, having the following elements in the 5′ to 3′ direction, i) a promoter, ii) DNA encoding a DNA binding domain of the bacterial repressor LexA, iii) DNA encoding a transactivating domain of VP16, iv) DNA encoding the regulatory domain of an estrogen receptor.
An isolated nucleic acid encoding a transcription factor, comprising, in the 5′ to 3′ direction, i) a promoter, ii) DNA encoding a DNA binding domain of the bacterial repressor LexA, iii) DNA encoding a transactivating domain of VP16, iv) DNA encoding a regulatory domain of an estrogen receptor.
Dependencies are inferred from the claim text.