Transgenic plants and plant cells comprising a reduced expression of invertase inhibitors
OwnerThomas Rauschinventor
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Abstract
The invention relates to transgenic plants and plant cells comprising a reduced expression of invertase inhibitors. The modification of the expression of the invertase inhibitors is achieved by introducing a cDNA sequence in an antisense orientation with respect to a promoter. The expression of the antisense DNA sequence is under the regulation of either the CaMV35S promoter or a tissue specific promoter.
Claims13
A process for producing a transgenic plant, whose seeds have an increased amount of reserve material in comparison with a wild-type plant due to the reduction or elimination of the expression of an endogenous invertase inhibitor protein during the development of seeds so that the activity of invertase, which is subject to a regulation by the invertase inhibitor protein, is increased during the development of seeds leading to an increased accumulation of reserve material in the seed, said process comprising the steps of:
- (a) obtaining a nucleotide sequence coding for the invertase inhibitor protein expressed during seed development in flowers with young ovules;
- (b) inserting the coding nucleotide sequence in a DNA construct in anti-sense orientation next to a promoter as a regulatory unit;
- (c) transforming a plant cell of a plant, from which the coding nucleotide sequence was obtained, with the DNA construct;
- and
- (d) cultivating the plant cell and regenerating a plant, wherein the expression of the endogenous invertase inhibitor protein is reduced or eliminated during seed development.
Dependencies are inferred from the claim text.