Polynucleotide and polypeptide sequences of monkey cathepsin S
AssigneeOrtho-McNeil Pharmaceutical, Inc.
InventorsRobin Thurmond, Lars Karlsson, Sherry Baker
Life of this patent
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Abstract
DNAs encoding monkey cathepsin S have been cloned and characterized. The recombinant protein is capable of forming biologically active protein. The cDNA's have been expressed in recombinant host cells that produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified.
Claims8
An isolated and purified nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:
- 1.
An expression vector comprising a nucleic acid sequence set forth in SEQ ID NO:
- 1.
A substantially pure monkey Cathepsin S protease comprising the amino acid sequence set forth in SEQ ID NO:2.
A method of identifying compounds that inhibit monkey Cathepsin S protease activity, comprising:
- a) combining a compound suspected of being an inhibitor of monkey Cathepsin S protease activity with the monkey Cathepsin S protease having the amino acid sequence set forth in SEQ ID NO:2;
- and
- b) measuring an effect of the compound on the protease activity of the monkey Cathepsin S protease,
- whereby an inhibitory compound is identified by a reduction in the protease activity of the monkey Cathepsin S protease.
Dependencies are inferred from the claim text.