Secretory membrane protein that induces osteoblast differentiation
OwnerNaoki Kimura, Tomoko Toyoshimainventors
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Abstract
Three genes encoding secretory membrane proteins have been successfully isolated from an osteoblast-like cell line by a method for specifically cloning secretory membrane proteins. One of these genes encodes a novel receptor protein. The protein has only the extracellular region, binds to the cell membrane via a GPI anchor, and carries therein a cysteine-rich, repetitive region commonly conserved in the TNF receptor super family. Overexpression of this protein in osteoblast-like cell line cells suppresses cell proliferation, changes cell morphology, and increases alkaline phosphatase activity, which is one of markers for differentiation of osteoblasts.
Claims27
A substantially pure polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO:2, wherein the polypeptide induces differentiation of an osteoblast.
A substantially pure polypeptide comprising SEQ ID NO:2.
A substantially pure polypeptide comprising SEQ ID NO:1.
A substantially pure polypeptide comprising the amino acid sequence of SEQ ID NO:2 containing up to 30 conservative amino acid substitutions, wherein the polypeptide induces differentiation of an osteoblast.
A substantially pure polypeptide encoded by a first nucleic acid that hybridizes under stringent conditions (0.2× SSC and 0.1% SDS at 68° C.) to a second nucleic acid consisting of SEQ ID NO:3, wherein the polypeptide induces differentiation of an osteoblast.
A substantially pure polypeptide consisting of the sequence of SEQ ID NO:1.
A substantially pure polypeptide consisting of the sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.