Loading patent…
AssigneeThe Rockefeller University
InventorsH. Robert Masure, Carsten I. Rosenow, Elaine Tuomanen, Theresa M. Wizemann
Estimating the term…
The invention relates to bacterial choline binding proteins (CBPs) which bind choline. Such proteins are particularly desirable for vaccines against appropriate strains of Gram positive bacteria, particularly streptococcus, and more particularly pneumococcus. Also provided are DNA sequences encoding the bacterial choline binding proteins or fragment thereof, antibodies to the bacterial choline binding proteins, pharmaceutical compositions comprising the bacterial choline binding proteins, antibodies to the bacterial choline binding proteins suitable for use in passive immunization, and small molecule inhibitors of choline binding protein mediated adhesion. Methods for diagnosing the presence of the bacterial choline binding protein, or of the bacteria, are also provided. In a specific embodiment, a streptococcal choline binding protein is an enolase, which demonstrates strong affinity for fibronectin.
An isolated nucleic acid encoding a streptococcal choline binding protein;
An oligonucleotide capable of screening for a nucleic acid encoding a streptococcal choline binding protein, wherein the oligonucleotide or its complement encodes at least 5 continuous amino acids of SEQ ID NO:
An isolated nucleic acid encoding a streptococcal choline binding protein comprising the amino acid sequence of SEQ ID NO:25 or SEQ ID NO:25 comprising a conservative amino acid substitution;
A nucleic acid that has at least 75% identity to the nucleotide sequence of SEQ ID NO:
A nucleic acid that has at least 80% identity to the nucleotide sequence of SEQ ID NO:
A nucleic acid that has at least 90% identity to the nucleotide sequence of SEQ ID NO:
A nucleic acid that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
Dependencies are inferred from the claim text.