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US 6,784,159 B2Granted
Triterpene saponins from soybeans for treating kidney disease
AssigneesHer Majesty the Queen in right of Canada, as represented by the Minister of Agriculture, University of Guelph
InventorsBruce J. Holub, F. William Collins, Dominique P. Bureau, Diana J. Philbrick
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Abstract
A method of treating polycystic kidney disease is described. The method involves administering soyasaponin Bb to an animal in need thereof.
Claims8
A method of treating polycystic kidney disease comprising administering a composition comprising an effective amount of isolated end purified soyasaponin Bb as the sole active ingredient to an animal in need thereof.
A method of isolating soyasaponin Bb from a sample comprising:
- (a) solubilizing the sample in acidified aqueous alcohol;
- (b) removing polar lipids by liquid chromatography;
- (c) solubilizing the sample from (b) in aqueous alcohol;
- (d) passing the sample from (c) through an anion exchange column;
- (e) eluting the sample absorbed to column in (d) with an acidified aqueous alcohol;
- and
- (f) purifying the sample from (e) by liquid chromatography by passing the sample through a preparative hydrophobic interaction chromatographic column comprising an electrostatically-linked, aliphatic- or alicyclic-substituted anionic or cationic polysaccharide gel and collecting fractions containing soyasaponin Bb.
4 more elements
Dependencies are inferred from the claim text.