Inhibitor and stimulator of stem cell proliferation and uses thereof
AssigneeWellstat Therapeutics Corporation
InventorsStephen D. Wolpe, Irena Tsyrlova
Life of this patent
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Abstract
Disclosed and claimed are methods for the isolation and use of stem cell modulating factors for regulating stem cell cycle and for accelerating the post-chemotherapy peripheral blood cell recovery. Also disclosed and claimed are the inhibitors and stimulators of stem cell proliferation.
Claims15
A method of stimulating stem cell proliferation comprising contacting hematopoietic cells with a stem cell proliferation stimulating amount of INPROL or an opiate compound or a stem cell proliteration stimulating amount of a combination of INPROL and an opiate compound,
- wherein said INPROL is selected from the group consisting of
- a polypeptide having the sequence of amino acids 1-97 of the human alpha hemoglobin chain,
- a polypeptide having the sequence of amino acids 1-94 of the human alpha hemoglobin chain,
- Phe-Pro-His-Phe-Asp-Leu-Ser-His-Gly-Ser-Ala-Gln-Val (SEQ ID NO:1),
- Cys-Phe-Pro-His-Phe-Asp-Leu-Ser-His-Gly-Ser-Ala-Gln-Val-Cys (SEQ ID NO:2)
- (where the two Cys residues form a disulfide bond),
- Asp-Ala-Leu-Thr-Asn-Ala-Val-Ala-His-Val-Asp-Asp-Met-Pro-Asn-Ala-Leu-Ser-Ala (SEQ ID NO:3),
- Phe-Leu-Gly-Phe-Pro-Thr (SEQ ID NO:33),
- Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe (SEQ ID NO:4),
- Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg (SEQ ID NO:5),
- Leu-Val-Val-Tyr-Pro-Trp-Thr (SEQ ID NO:6),
- Leu-Val-Val-Tyr-Pro-Trp-Thr (SEQ ID NO:7),
- Leu-Val-Val-Tyr-Pro-Trp (SEQ ID NO:8).
- Leu-Val-Val-Tyr-Pro (SEQ ID NO:9),
- Val-Val-Tyr-Pro-Trp-Thr-Gln (SEQ ID NO:10),
- Tyr-Pro-Trp-Thr-Gln-Arg-Phe (SEQ ID NO:11),
- Tyr-Pro-Trp-Thr-Gln-Arg (SEQ ID NO:12),
- Tyr-Pro-Trp-Thr-Gln (SEQ ID NO:13), and
- Tyr-Pro-Trp-Thr (SEQ ID NO:27):
- wherein said stem cells are cells which can generate multiple lineages or other stem cells.
The method of clam 3, comprising contacting hematopoietic cells with a stem cell proliferation stimulating amount of Cys-Phe-Pro-His-Phe-Asp-Leu-Ser-His-Gly-Ser-Ala-Gln-Val-Cys (SEQ ID NO:2) wherein the two Cys residues form a disulfide bond.
A method of stimulating stem cell proliferation comprising contacting hematopoietic cells with a stem cell proliferation stimulating amount of a compound capable of binding opiate receptors, wherein said stem cells are cells which can generate multiple lineages or other stem cells.
A method of stimulating stem cell proliferation comprising contacting stem cells with a stem cell proliferation stimulating amount of INPROL or an opiate compound or a stem cell proliferation stimulating amount of a combination at INPROL and an opiate compound,
- wherein sad INPROL is selected from the group consisting of
- a polypeptide having the sequence of amino acids 1-97 of the human alpha hemoglobin chain,
- a polypeptide having the sequence of amino acids 1-94 of the human alpha hemoglobin chain,
- Phe-Pro-His-Phe-Asp-Leu-Ser-His-Gly-Ser-Ala-Gln-Val (SEQ ID NO:1),
- Cys-Phe-Pro-His-Phe-Asp-Leu-Ser-His-Gly-Ser-Ala-Gln-Val-Cys (SEQ ID NO:2)
- (where the two Cys residues form a disuffide bond),
- Asp-Ala-Leu-Thr-Asn-Ala-Val-Ala-His-Val-Asp-Asp-Met-Pro-Asn-Ala-Leu-Ser-Ala (SEQ ID NO:3),
- Phe-Leu-Gly-Phe-Pro-Thr (SEQ ID NO:33),
- Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe (SEQ ID NO:4),
- Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg (SEQ ID NO:5),
- Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln (SEQ ID NO:6),
- Leu-Val-Val-Tyr-Pro-Trp-Thr (SEQ ID NO:7),
- Leu-Val-Val-Tyr-Pro-Trp (SEQ ID NO:8),
- Leu-Val-Val-Tyr-Pro (SEQ ID NO:9),
- Val-Val-Tyr-Pro-Trp-Thr-Gln (SEQ ID NO:10),
- Tyr-Pro-Trp-Thr-Gln-Arg-Phe (SEQ ID NO:11),
- Tyr-Pro-Trp-Thr-Gln-Arg (SEQ ID NO:12),
- Tyr-Pro-Trp-Thr-Gln (SEQ ID NO:13), and
- Tyr-Pro-Trp-Thr (SEQ ID NO:27):
- wherein said stem cells are cells which can generate multiple lineages or other stem calls.
Dependencies are inferred from the claim text.