Method for obtaining components from cultured leucocyte
OwnerTsukasa Matsumotoinventor
Life of this patent
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Abstract
A method for obtaining leucocyte components from human blood including (A) a first step for fracturing the cell membrane of leucocytes of human blood by using a freezing and defrosting method, a supersonic application, laser irradiation, an osmotic pressure changing method, a vacuum chamber, or the like and (B) a second step for separating and collecting the leucocyte-components containing the leucocytes with fractured cell membranes from the blood liquid resulted from the first step by way of a centrifugal precipitation technique or an electrophoresis technique. After being separated and collected, the leucocyte components are, respectively, subjected to various therapeutic tests using blood samples collected from patients suffering from various diseases to determine the therapeutic effects.
Claims8
A method for obtaining leucocyte components from human blood comprising;
- (A) a first step for fracturing cell membranes of leucocytes of human blood by physical means, and
- (B) a second step for separating leucocyte components from blood liquid resulting from the first step, said blood liquid containing leucocytes with fractured cell membranes, in order so that separated layers or parts can be collected individually;
- and further comprising a step for determining therapeutic effects of separated and collected leucocyte components, said step including combining isolated leucocyte components with blood cells obtained from a patient with a pro-determined disease and thereafter observing effects of said isolated leucocyte components when combined with said blood cells.
A method of identifying a therapeutically effective leucocyte component comprising:
- fracturing cell membranes of leucocytes cultured from healthy human blood by physical means and isolating leucocyte components obtained therefrom;
- obtaining blood cells from a patient with a pre-determined disease and separating said blood cells into upper layer blood cells and lower layer blood cells;
- dividing each of said upper layer blood cells and lower layer blood cells into a plurality of test samples;
- adding said isolated leucocyte components to said plurality of test samples thereby to determine the therapeutic effect of each said isolated leucocyte component on said blood cells;
- and
- selecting a leucocyte component therapeutically effective for treating said pre-determined disease by identifying the component which, when added to said test samples, results in the least degeneration of erythrocytes and longest erythrocyte life span.
Dependencies are inferred from the claim text.