Process for the cryo-preservation of plants
AssigneeNestec S.A.
InventorsBruno Florin, Vincent Petiard
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Abstract
An improved process for the cryo-preservation of plants, especially for the direct cryo-preservation of plant tissues. This process includes the steps of dehydrating, pre-freezing and cryo-freezing plant derived tissue, with the improvement being that the plant tissue to be cryo-preserved is a primary explant that has been subjected an induction treatment for regeneration.
Claims11
A process for the cryo-preservation of a primary regeneration tissue derived from cocoa plant or a coffee plant comprising the following steps:
- cultivating a plant tissue derived from cocoa plant or a coffee plant on an induction medium for a time sufficient to induce a primary regeneration tissue comprising embryogenic cells;
- culturing the primary regenerating tissue on a multiplication medium for a time sufficient to maintain a stable proliferation of the primary regeneration tissue;
- treating the primary regeneration tissue in a two step process that comprises sequential incubation in first and second sucrose media, wherein the second medium contains a greater amount of sucrose than the first medium;
- prefreezing the primary regenerating tissue to a temperature between −20° C. and −40° C.;
- and
- cryofreezing the primary regeneration tissue.
A process for the cryo-preservation of a primary regeneration tissue derived from a cocoa plant or a coffee plant comprising the steps of:
- incubating a plant tissue derived from a cocoa plant or a coffee plant in an induction medium for a time sufficient to induce a primary regeneration tissue comprising embryogenic cells;
- pretreating the primary regeneration tissue by culturing the primary regeneration tissue on successive culture media with an increasing concentration of sucrose;
- and
- cryofreezing the primary regeneration tissue.
Dependencies are inferred from the claim text.