Loading patent…
AssigneeInstitut National De La Sante Et De La Recherche Medicale (Inserm)
InventorsMarcelo Lopez Lastra, Caroline Gabus-Darlix, Jean-Luc Darlix
Estimating the term…
The invention concerns the use of a nucleotide sequence derived from all or part of the genomic RNA 5′ end of a type C retrovirus except for Friend murine leukaemia virus (FMLV) and Moloney murine leukaemia virus (MoMLV) as internal ribosome entry site or as element enabling or improving retrovirus vector packaging. The invention also concerns a vector comprising said nucleotide sequence, a viral particle generated from this vector, a cell comprising this vector or infected by the viral particle, their therapeutic use and a pharmaceutical composition containing them. The invention further concerns the use of a vector, a viral particle or a pharmaceutical composition for transfecting or infecting pluripotent stem cells.
A vector for the expression of one or more genes of interest comprising a nucleotide sequence isolated from the 5′ end of the genomic RNA of a an avian reticuloendotheliosis virus of type A (REV-A), wherein said nucleotide sequence comprises at least the portion of the sequence presented in SEQ ID NO:
A method for providing an internal ribosome entry site (IRES) to a vector for the transfer and expression of one or more genes of interest, comprising the step of introducing into said vector a nucleotide sequence isolated from the 5′ end of the genomic RNA of an avian reticuloendotheliosis virus of type A (REV-A) wherein said nucleotide sequence comprises at least the portion of the sequence presented in SEQ ID NO:
A method of allowing or activating the encapsidation of a retrovirus or of a retroviral vector, comprising the step of introducing into said retrovirus or retroviral vector, a nucleotide sequence isolated from the 5′ end of the genomic RNA of a reticuloendotheliosis virus of type A (REV-A), wherein said nucleotide sequence comprises at least the portion of the sequence presented in SEQ ID NO:
Dependencies are inferred from the claim text.