Human glycosylation enzymes
AssigneesHuman Genome Sciences, Inc., The Johns Hopkins University
InventorsTimothy A. Coleman, Michael J. Betenbaugh
Life of this patent
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Abstract
The present invention relates to novel human glycosylation enzyme polypeptides and isolated nucleic acids containing the coding regions of the genes encoding such polypeptides. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human glycosylation enzyme polypeptides. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human glycosylation enzyme polypeptides.
Claims118
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding amino acid residues 1 to 434 of SEQ ID NO:2;
- and
- (b) a polynucleotide encoding amino acid residues 2 to 434 of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding the amino acid sequence of the full-length CMP Sialic Acid Synthetase (CMP-SAS) polypeptide, which amino acid sequence is encoded by the HWLLM34 cDNA clone contained in ATCC Deposit No. PTA-1410;
- and
- (b) a polynucleotide encoding the amino acid sequence of the full-length CMP-SAS polypeptide, excluding the N-terminal methionine residue, which amino acid sequence is encoded by the HWLLM34 cDNA clone contained in ATCC Deposit No. PTA-1410.
An isolated nucleic acid molecule comprising a polynucleotide encoding a first polypeptide 90% or more identical to a second polypeptide selected from the group consisting of:
- (a) amino acid residues 1 to 434 of SEQ ID NO:2;
- and
- (b) amino acid residues 2 to 434 of SEQ ID NO:2,
- wherein said first polypeptide has CMP-SAS activity or specifically binds an antibody that specifically binds the polypeptide of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a polynucleotide encoding a first polypeptide 90% or more identical to a second polypeptide selected from the group consisting of:
- (a) the full-length CMP-SAS polypeptide as encoded by the HWLLM34 cDNA clone contained in ATCC Deposit No. PTA-1410;
- and
- (b) the full-length CMP-SAS polypeptide, excluding the N-terminal methionine residue as encoded by the HWLLM34 cDNA clone contained in ATCC Deposit No. PTA-1410,
- wherein said first polypeptide has CMP-SAS activity or specifically binds an antibody that specifically binds the polypeptide of SEQ ID NO:2.
An isolated nucleic acid molecule comprising a polynucleotide fragment encoding a fragment of SEQ ID NO:2 with CMP-SAS.
An isolated nucleic acid molecule comprising a polynucleotide fragment encoding a fragment of the polypeptide encoded by the HWLLM34 cDNA clone contained in ATCC Deposit No. PTA-1410, wherein said fragment has CMP-sialic acid synthetase activity.
A nucleic acid fragment comprising a polynucleotide encoding a fragment of SEQ ID NO:2, having CMP-SAS activity.
A nucleic acid fragment comprising a polynucleotide encoding a fragment of the polypeptide having CMP-SAS activity encoded by the HWLLM34 cDNA clone contained in ATCC Deposit No. PTA-1410, wherein said polynucleotide is operatively associated with a regulatory element to direct expression of said polypeptide fragment.
An isolated polynucleotide comprising a polynucleotide which hybridizes to the complement of the polynucleotide set forth in SEQ ID NO:1 wherein under conditions of hybridization in a buffer consisting of 50% formamide, 5× SSC, 50 mM sodium phosphate (pH 7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA at 42° C. and wash in a solution consisting of 0.1× SSC at 65° C.;
- wherein said polynucleotide encodes a polypeptide with CMP-sialic acid synthetase activity.
An isolated polynucleotide comprising a polynucleotide which hybridizes to the complement of the CMP-sialic acid synthetase coding sequence of the HWLLM34 cDNA clone contained in ATCC Deposit No. PTA-1410 under conditions of hybridization in a buffer consisting of 50% formamide, 5× SSC, 50 mM sodium phosphate (pH 7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA at 42° C. and wash in a solution consisting of 0.1× SSC at 65° C.;
- wherein said polynucleotide encodes a polypeptide with CMP-sialic acid synthetase activity.
Dependencies are inferred from the claim text.