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AssigneeUpFront Chromatography
InventorsMorten Aae Olander, Allan Otto Fog Lihme, Timothy John Hobley, Marcos Simon, Irini Theodossiou, Owen Robert Tyrynis Thomas
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The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 μm, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
A particulate material having a density of at least 2.5 g/ml, comprised of particles having an average diameter of 5-75 μm, wherein said particles of the particulate material are essentially constructed of a polymeric base matrix and a non-porous core material, said core material having a density of at least 3.0 g/ml, and said polymeric base matrix comprising either chargeable pendant groups or affinity ligands for a bio-molecule.
A particulate material having a density of in the range of 3.2-5.0 g/ml, comprised of particles having an average diameter of 15-45 μm, and the particles of the particulate material are essentially constructed of a polysaccharide base matrix and a core material, said core material having a density in the range of 6.0-12.0 g/m3, said polysaccharide base matrix including pendant groups selected from polyethyleneimine chains, modified polyethyleneimine chains and poly(ethyleneimine/oxyethylene) chains, said pendant groups forming a tentacular structure on the surface of the particle.
Dependencies are inferred from the claim text.