Expressed sequence tags and encoded human proteins

A method of making a cDNA comprising the steps of:

• (i) contacting a collection of mRNA molecules from human cells with a first primer capable of hybridizing to the polyA tail of said mRNA;
• (ii) hybridizing said first primer to said polyA tail;
• (iii) reverse transcribing said mRNA to make a first cDNA strand;
• (iv) making a second cDNA strand complementary to said first cDNA strand using at least one primer comprising at least 15 consecutive nucleotides of a sequence SEQ ID NO:
• 3792;
• and
• (v) isolating the resulting cDNA comprising said first cDNA strand and said second cDNA strand;
• wherein the second cDNA strand is made by:
• (i) contacting said first cDNA strand with a first pair of primers, said first pair of primers comprising a second primer comprising at least 15 consecutive nucleotides of a sequence of SEQ ID NO:
• 3792 and a third primer having a sequence therein which is included within the sequence of said first primer;
• (ii) performing a first polymerase chain reaction with said first pair of primers to generate a first PCR product;
• (iii) contacting said first PCR product with a second pair of primers, said second pair of primers comprising a fourth primer, said fourth primer comprising at least 15 consecutive nucleotides of said sequence of SEQ ID NO:
• 3792, and a fifth primer, wherein said fourth and fifth hybridize to sequences within said first PCR product;
• and
• (iv) performing a second polymerase chain reaction, thereby generating a second PCR product.